解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::The role of platelet-derived growth factor (PDGF) in the control of smooth muscle cell (SMC) differentiation was explored in vitro by examining its effects on expression of the smooth muscle (SM) specific contractile protein SM alpha actin in cultured rat aortic SMC. Quiescent, postconfluent SMC express maximal levels...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041420325
更新日期:1990-03-01 00:00:00
abstract::During the early period after poliovirus infection of HeLa cells, cellular Na+/K+ ATPase activity is transiently activated. We investigated the possibility that Na+/K+ ATPase activation is a consequence of Na+/H+ antiporter activation. Increased uptake of the weak organic acid 5,5-dimethyloxazolidine-2,4-dione by infe...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041420319
更新日期:1990-03-01 00:00:00
abstract::Terminal deoxynucleotidyl transferase (TdT) is a template-independent DNA polymerase that is transiently expressed during the normal development of T and B lymphocytes. Phorbol 12-myristate 13-acetate (PMA) has been reported to induce maturation-like changes, including the loss of TdT, in many leukemic cell lines. We ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041420311
更新日期:1990-03-01 00:00:00
abstract::Factors inhibiting cell growth have been isolated from different cell types. However, little information is available concerning their mode of action. A novel growth inhibitory factor of 45 kDa (IDF45) was recently purified to homogeneity from medium conditioned by 3T3 cells. This molecule was able to inhibit DNA synt...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041420219
更新日期:1990-02-01 00:00:00
abstract::Mouse L-929 cells were subjected to increasing concentrations of sorbitol, which remove cell water and reduce volume osmotically. The rate of lactate production from glucose was significantly higher in osmotically perturbed cells than in controls, both in monolayers and in suspensions. L cells can apparently use sorbi...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041420223
更新日期:1990-02-01 00:00:00
abstract::It was the goal of this study to determine whether during long-term quiescence WI-38 cells gradually lose labile components which then need to be resynthesized before a stimulated cell can progress through G-1 and enter S. The metabolic and molecular status of WI-38 cells was systematically analyzed as they entered an...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041420117
更新日期:1990-01-01 00:00:00
abstract::Sulfogalactosylglycerolipid (SGG) is the major mammalian male germ cell glycolipid and has been implicated in sperm/egg binding. Mycoplasma pulmonis, a species of Mollicutes, is associated with male infertility in rodents. Purified SGG incubated in the presence of M. pulmonis was enzymatically degraded by both desulfa...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041420121
更新日期:1990-01-01 00:00:00
abstract::Cells isolated from fetal rat calvaria (RC) and maintained in vitro in medium containing ascorbic acid and B-glycerophosphate form three-dimensional, mineralized nodules having the histological, immunohistological, and ultrastructural characteristics of woven bone. We have studied the effects of forskolin (FSK), a dit...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041420109
更新日期:1990-01-01 00:00:00
abstract::Normal FBAE AG 7680 cells and chemically transformed FBAE GM 7373 cells were compared for their capacity to produce and to respond to bFGF. Normal FBAE cells showed higher levels of bFGF protein and of poly(A)+ bFGF mRNA than transformed GM 7373 cells, indicating that chemical transformation in FBAE cells is parallele...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041410310
更新日期:1989-12-01 00:00:00
abstract::Hybridization analysis of mRNA with a cDNA probe for human sucrase-isomaltase, pulse-chase experiments with L-[35S]-methionine followed by SDS-PAGE, and immunofluorescence detection of sucrase-isomaltase were used to analyze the level(s) at which forskolin interferes with the expression of the enzyme in Caco-2 cells i...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041410322
更新日期:1989-12-01 00:00:00
abstract::Cerebroside sulfotransferase (EC 2.8.2.11, CST) specific activity has been determined in oligodendrocyte (OL)-enriched glial cell cultures from newborn rat brain grown in serum supplemented medium. This activity is detectable at 5 days in vitro (DIV) and reaches its maximum value at 12 DIV. This period corresponds to ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041410327
更新日期:1989-12-01 00:00:00
abstract::Synthesis of alpha- and beta-globin RNA in DMSO-induced Friend's erythroleukemia cells and synthesis of immunoglobulin gamma- and kappa-chain RNA, total RNA, 5S RNA, and tRNA in mouse myeloma cells (MPC-11) was inhibited by gamma-irradiation. For all RNA species, synthesis decreased nearly exponentially as a function ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041410216
更新日期:1989-11-01 00:00:00
abstract::Activation of T-lymphocytes by antigen, mitogenic lectins, or antibodies against the T-cell receptor complex, particularly in the presence of IL1, induces the secretion of the T-cell growth factor IL2. IL2 then has a major role in regulating the duration and magnitude of the immune response. Interaction of antigen, an...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041410212
更新日期:1989-11-01 00:00:00
abstract::We have analyzed the expression of Na,K-ATPase alpha subunit isoforms in the transporting ciliary processes of the human eye and in cultured cells derived from non-pigmented (NPE) and pigmented (PE) ciliary epithelium. Northern hybridization analysis shows that the mRNAs encoding all the three distinct forms of Na,K-A...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041410203
更新日期:1989-11-01 00:00:00
abstract::Guanine nucleotides and pertussis toxin were used to test for the involvement of a guanine nucleotide binding protein in the vasopressin V1 receptor-mediated stimulation of protein kinase C activity in Swiss 3T3 cells. Addition of vasopressin in the presence of [gamma-32P]ATP and digitonin caused a marked and rapid in...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041410204
更新日期:1989-11-01 00:00:00
abstract::Transforming growth factor beta type 1 (TGF-beta 1) was reacted with NHS-biotin to yield a derivative of TGF-beta 1 which was biotinylated on lysine residues. The biotinylated form of TGF-beta 1 was separated from the unreacted material by reverse phase chromatography. In three separate bioassays, the derivatized pept...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041410125
更新日期:1989-10-01 00:00:00
abstract::We have investigated the characteristics of a transport system in HeLa cells, which turned out to be very similar to a previously described Na+, K+, 2Cl- -cotransport system. For further understanding about the physiological role of the cotransporter, we have mutagenized HeLa cells and selected progeny cells for growt...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041410126
更新日期:1989-10-01 00:00:00
abstract::Human skin fibroblasts contracted fibrin gels in a time- and cell-dependent manner. Under optimal conditions, gel contraction amounted to more than 50% in 2 hr. Fibronectin did not promote contraction, and fibrinolysis was not required for contraction, although gels contracted without serum or aprotinin were lysed. Be...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041400324
更新日期:1989-09-01 00:00:00
abstract::The protein kinase C stimulator TPA (12-O-tetradecanoyl phorbol-13-acetate) enhanced the responsiveness of adenylate cyclase to IPR (isoproterenol) and PGE1 (prostaglandin E1) in quiescent tsKSV-NRK cells at the nonpermissive 41 degrees C. Reactivating the thermolabile mitogenic/oncogenic K-ras protein in tsKSV-NRK ce...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041400302
更新日期:1989-09-01 00:00:00
abstract::Addition of ATP to cultures of transformed mouse fibroblasts, 3T6 cells, resulted in cell growth inhibition, whereas the growth of the non-transformed counterparts, 3T3 cells, was only slightly affected. The inhibition was found to be specific for adenine nucleotides, and concentration dependent. At relatively low con...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041400318
更新日期:1989-09-01 00:00:00
abstract::The temperature-sensitive (ts) Chinese hamster ovary (CHO) cell mutant tsH1 contains a thermolabile leucyl-tRNA synthetase. Upon incubation at the nonpermissive temperature of 39.5 degrees C, the enzyme became reversibly inhibited over a period of minutes, and the cells lost viability over a period of many hours. Howe...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041400206
更新日期:1989-08-01 00:00:00
abstract::Human peripheral blood monocytes and neutrophils secrete TGF-beta. Activation of monocytes with LPS stimulates the secretion of TGF-beta; however, the production of TGF-beta by neutrophils was not altered by treatment with LPS. The secreted TGF-beta appears to be in a fully active form since acid treatment of the cond...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041400226
更新日期:1989-08-01 00:00:00
abstract::The human chronic myeloid leukemia cell line K562 acquires several megakaryoblastoid features when cultured in the presence of the tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate (TPA). We observed strongly increased secretion of several proteins into the culture media of K562 cells within a few hours of TPA trea...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041400115
更新日期:1989-07-01 00:00:00
abstract::The cellular basis of the membrane-limited state of glucose utilization and the mechanism of the endogenous regulation of hexose uptake in dense monolayers of C6 glioma cells were investigated. In an earlier study, it was shown that at high rates of glucose transport and phosphorylation combined with the inhibition of...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041400105
更新日期:1989-07-01 00:00:00
abstract::Normal human keratinocytes (NHK) were cultured in serum-free medium, containing low (0.1 mM) or high (2 mM) calcium, to obtain proliferating and differentiating cultures, respectively. Proteoglycan (PG) synthesis of proliferating and differentiating NHK was investigated. Cultures were labeled with 35S-sulfate, and the...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041400113
更新日期:1989-07-01 00:00:00
abstract::This study describes the alterations induced by Interleukin-1 alpha and -beta (IL-1 alpha and IL-1 beta) on fibroblast-synthesized extracellular matrix. Fibroblasts were grown between pieces of dentin or in collagen-coated Terasaki wells for 3 or 6-9 weeks to create 3-dimensional cell-containing matrices constituted p...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041390308
更新日期:1989-06-01 00:00:00
abstract::We have examined the ability of epidermal growth factor (EGF) to regulate prostacyclin production by cultured A10 smooth muscle cells. EGF by itself had no effect on prostacyclin production, but it augmented the response to arg8-vasopressin. An AGF stimulation of prostacyclin production was also observed in the presen...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041390311
更新日期:1989-06-01 00:00:00
abstract::Considerable evidence has accumulated for a role of a nonsteroidal mevalonate product in the regulation of DNA replication and cell division. We report here a similar requirement for mevalonate in a nonreplicative function, that of natural killer (NK) cell cytotoxicity. Treatment of NK cells with 10 microM compactin f...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041390314
更新日期:1989-06-01 00:00:00
abstract::NIH-3T3 cells transformed by the EJ-ras oncogene display reduced platelet-derived growth factor (PDGF)-stimulated phospholipase C activity as measured by inositol 1,4,5-triphosphate (IP3) synthesis and Ca2+ mobilization. The lack of PDGF-stimulated Ca2+ mobilization in EJ-ras transformed cells is not due to a loss of ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041390216
更新日期:1989-05-01 00:00:00
abstract::Protein-DNA interactions within the promoter of a cell cycle-regulated human H4 histone gene were examined by binding of 5'-end-labeled DNA segments to Western blots of nuclear protein fractions. Specific protein interactions were observed with DNA segments located between -500 bp and -1,070 bp upstream of the ATG ini...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041390214
更新日期:1989-05-01 00:00:00
abstract::The effect of retinoic acid on human fibroblasts was studied in a cell culture model of chronologic aging and photoaging. During early exponential phase, all trans-retinoic acid significantly stimulated growth rate of adult arm-derived dermal fibroblasts but not of newborn or adult foreskin-derived fibroblasts. Retino...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041390117
更新日期:1989-04-01 00:00:00
abstract::Biochemical events elicited by interleukin 1 (IL-1) were studied in Swiss 3T3 fibroblasts. One hour after its addition, IL-1 stimulated synthesis of prostaglandin E2 (PGE2), which continued to accumulate for 4 days. IL-1 also stimulated cAMP accumulation. Indomethacin blocked cAMP accumulation in response to IL-1, sug...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041390106
更新日期:1989-04-01 00:00:00
abstract::Properties of the change from asparagine dependence (asn-) to independence (asn+) were investigated in the androgenetic haploid frog cell line ICR 2A. Two types of asn+ variants arose spontaneously during culture. Glutamine-dependent asparagine synthetase (AS) activity, found to be deficient in asn- cells, was repress...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041390124
更新日期:1989-04-01 00:00:00
abstract::Subcellular localization of the large multicatalytic protease complexes called proteasomes, which have been found in soluble fractions of various cells, was examined by biochemical, immunological, and immunohistological methods. Rat liver nuclei, purified by two different procedures, showed high activities for degradi...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041390107
更新日期:1989-04-01 00:00:00
abstract::We have utilized the in situ perfused rat liver under nonrecirculating conditions to examine the effect of temperature on the metabolism and biliary secretion of [125I]-asialoorosomucid (ASOR). In this manner we were able to follow the fate of a single round of internalized ligand. In control livers perfused at 37 deg...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041380316
更新日期:1989-03-01 00:00:00
abstract::We report the continuous growth maintenance of untransformed and chemically transformed fibroblasts (AKR-2B, AKR-MCA cells) in low concentrations of serum (0.1% FBS). The cell lines established (AKR-0.1F, MCA-0.1F) proliferated at rates comparable to cells maintained under high serum conditions (10% FBS). Complete rem...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041380303
更新日期:1989-03-01 00:00:00
abstract::The mechanism(s) by which heparin influences the biological activities of acidic and basic fibroblast growth factors (aFGF and bFGF) is not completely understood. One mechanism by which heparin could alter the biological activities of aFGF and bFGF is by altering their biological half-lives. We investigated the possib...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041380202
更新日期:1989-02-01 00:00:00
abstract::Cultured rat embryo cells were stimulated to rapidly release a small group of proteins that included several heat-shock proteins (hsp110, hsp71, hscp73) and nonmuscle actin. The extracellular proteins were analyzed by two-dimensional polyacrylamide gel electrophoresis. Heat-shocked cells released the same set of prote...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041380206
更新日期:1989-02-01 00:00:00
abstract::A basic understanding of the recruitment of quiescent tumor cells into the cell cycle would be an important contribution to tumor biology and therapy. As a first step in pursuing this goal, we have investigated the regrowth kinetics of cells from different regions in multicellular spheroids of rodent and human origin....
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041380222
更新日期:1989-02-01 00:00:00
abstract::We have studied the tissue distribution of interleukin (IL) and hemopoietic colony-stimulating factor (CSF) transcripts in mice by S1-nuclease protection analysis. Accumulation of several of these mRNAs in response to intravenous injection of lipopolysaccharide (LPS) appears to occur in a tissue-specific fashion. IL-1...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041380107
更新日期:1989-01-01 00:00:00